Glucose and calcium ions may modulate the efficiency of bovine B-casomorphin-7 permeability through a monolayer of Caco-2 cells
| Autor: | Ewa Fiedorowicz, Elżbieta Kostyra, Michał Matysiewicz, Edyta Sienkiewicz-Szłapka, Malgorzata Teodorowicz, Beata Jarmołowska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cell Membrane Permeability
tight junctions Physiology chemistry.chemical_element Enzyme-Linked Immunosorbent Assay Celbiologie en Immunologie Calcium Biochemistry Cellular and Molecular Neuroscience Endocrinology opioid-peptides Monolayer medicine Animals Humans milk Intestinal permeability Chemistry intestinal permeability Biological activity medicine.disease gene-expression Peptide Fragments beta-casomorphin Glucose Membrane Cell Biology and Immunology iv Caco-2 Permeability (electromagnetism) transport WIAS endothelial permeability Cattle Endorphins Casomorphin Caco-2 Cells absorption |
| Zdroj: | Peptides 49 (2013) Peptides, 49, 59-67 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2013.08.017 |
| Popis: | Milk and dairy products provide a lot of valuable nutritive elements. They are also sources of biologically active peptides, including ß-casomorphins that manifest the properties of morphine. An activity of DPPIV seems to be most crucial factor decreasing the efficiency of the ß-casomorphin-7 (BCM7) transport. The increase of BCM7 concentration in blood may intensify symptoms of apparent life threatening events (ALTE), autism, schizophrenia, and allergy. This study aimed at identifying the influence of several selected substances on a transport efficiency of bovine BCM7 through an intestinal monolayer in a Caco-2 cell model system. Applying the ELISA method, the permeability coefficient of BCM7 through the Caco-2 monolayer was calculated. TEER values were used to evaluate the integrity of Caco-2 cell monolayers. An increase of glucose and Ca(2+) concentrations in the culture medium was accompanied by an increase of the BCM7 transport efficiency. The lowest permeability coefficients of BCM7 were observed for the membranes with high electrical resistances. The transport was enhanced in the presence of milk infant formulas, whereas no changes were observed when using µ-opioid receptor antagonist (casoxin-6). The results may be useful in understanding the pathogenesis of inflammation and food allergy in infants. |
| Databáze: | OpenAIRE |
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