Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility
Autor: | Eric J. Brown, J. Hiroshi Morisaki, Sunao Takeshita, Kent L. McDonald, Lian-Yong Gao, Matthew D. Welch, Luisa M. Stamm, John E. Heuser, Robert L. Jeng, Robyn Roth |
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Rok vydání: | 2003 |
Předmět: |
animal structures
mycobacteria Immunology Motility Mice Inbred Strains macrophage WASP macromolecular substances medicine.disease_cause Article Microbiology Mice 03 medical and health sciences Biopolymers Listeria monocytogenes Phagosomes medicine Animals Immunology and Allergy Cytoskeleton Mycobacterium marinum Actin 030304 developmental biology 0303 health sciences biology 030306 microbiology Intracellular parasite VASP bacterial infections and mycoses biology.organism_classification Actins 3. Good health Cell biology Cytoplasm bacteria Host cytoskeleton Arp2/3 |
Zdroj: | The Journal of Experimental Medicine |
ISSN: | 1540-9538 0022-1007 |
Popis: | Mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. Numerous intracellular pathogens, including Listeria monocytogenes, Shigella flexneri, and Rickettsia rickettsii, exploit the host cytoskeleton by using actin-based motility for cell to cell spread during infection. Here we show that Mycobacterium marinum, a natural pathogen of fish and frogs and an occasional pathogen of humans, is capable of actively inducing actin polymerization within macrophages. M. marinum that polymerized actin were free in the cytoplasm and propelled by actin-based motility into adjacent cells. Immunofluorescence demonstrated the presence of host cytoskeletal proteins, including the Arp2/3 complex and vasodilator-stimulated phosphoprotein, throughout the actin tails. In contrast, Wiskott-Aldrich syndrome protein localized exclusively at the actin-polymerizing pole of M. marinum. These findings show that M. marinum can escape into the cytoplasm of infected macrophages, where it can recruit host cell cytoskeletal factors to induce actin polymerization leading to direct cell to cell spread. |
Databáze: | OpenAIRE |
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