Transcriptional regulation ofNostocuptake hydrogenase
| Autor: | Rikard Axelsson, Fredrik Oxelfelt, Peter Lindblad |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Cyanobacteria Nostoc Hydrogenase Transcription Genetic Nitrogen Molecular Sequence Data Microbiology Bacterial Proteins Ammonia Transcription (biology) Genetics Molecular Biology Heterocyst chemistry.chemical_classification Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Anabaena Gene Expression Regulation Bacterial biology.organism_classification Blotting Southern Enzyme Biochemistry chemistry Genes Bacterial Oxidoreductases Hydrogen Anabaena variabilis |
| Zdroj: | FEMS Microbiology Letters. 170:77-81 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | Filamentous, heterocystous cyanobacteria may contain both an uptake hydrogenase (encoded by hupSL) and a bidirectional enzyme (encoded by hoxFUYH). The present study identifies three strains (Anabaena variabilis, Nostoc muscorum and Nostoc sp. strain PCC 73102) with a contiguous hupL in both vegetative cells and heterocysts. The two Nostoc strains differ in either containing a bidirectional enzyme (N. muscorum) or lacking this enzyme (N. PCC 73102). Transcriptional studies, using reverse transcriptase-polymerase chain reaction, demonstrated an induction of a hupL transcript approximately 24 h after a shift from non-nitrogen-fixing to nitrogen-fixing conditions (in parallel with the induction of an in vivo light-dependent H2-uptake activity) in N. muscorum. However, the level of hoxH transcripts did not change significantly during the induction of the H2-uptake activity. |
| Databáze: | OpenAIRE |
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