Molecular Basis for the Nerve Dependence of Limb Regeneration in an Adult Vertebrate
| Autor: | Anoop Kumar, James W. Godwin, Jeremy P. Brockes, Phillip B. Gates, Acely Garza-Garcia |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Glycosylphosphatidylinositols
medicine.medical_treatment AGR2 CD59 Antigens Biology Ligands Article Mice Two-Hybrid System Techniques Chlorocebus aethiops Notophthalmus viridescens medicine Animals Humans Regeneration Peripheral Nerves Growth Substances Cells Cultured Denervation Multidisciplinary Epidermis (botany) urogenital system Stem Cells Regeneration (biology) Growth factor Electroporation Extremities Anatomy Salamandridae Cell biology body regions COS Cells Intercellular Signaling Peptides and Proteins Stem cell Blastema |
| Zdroj: | Science. 318:772-777 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.1147710 |
| Popis: | The limb blastemal cells of an adult salamander regenerate the structures distal to the level of amputation, and the surface protein Prod 1 is a critical determinant of their proximodistal identity. The anterior gradient protein family member nAG is a secreted ligand for Prod 1 and a growth factor for cultured newt blastemal cells. nAG is sequentially expressed after amputation in the regenerating nerve and the wound epidermis—the key tissues of the stem cell niche—and its expression in both locations is abrogated by denervation. The local expression of nAG after electroporation is sufficient to rescue a denervated blastema and regenerate the distal structures. Our analysis brings together the positional identity of the blastema and the classical nerve dependence of limb regeneration. |
| Databáze: | OpenAIRE |
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