Development of a method for the determination of hydromorphone in plasma by LC-MS
| Autor: | Joan Bergman, Sherry K. Cox, Shawna Hawkins, Kurt K. Sladky |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
040301 veterinary sciences
Formic acid Clinical Biochemistry Mass spectrometry 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry 0403 veterinary science chemistry.chemical_compound Drug Stability Limit of Detection Liquid chromatography–mass spectrometry Drug Discovery medicine Humans Hydromorphone Solid phase extraction Acetonitrile Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Solid Phase Extraction 010401 analytical chemistry Extraction (chemistry) Reproducibility of Results 04 agricultural and veterinary sciences General Medicine 0104 chemical sciences Standard curve chemistry Linear Models medicine.drug |
| Zdroj: | Biomedical Chromatography. 32:e4357 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.4357 |
| Popis: | A simple high-performance liquid chromatography method for the determination of hydromorphone in small volume plasma has been developed. Following solid-phase extraction using Oasis HLB cartridges, samples were separated by reverse-phase high-performance liquid chromatography on an Atlantis T3 4.6 × 150 mm column (3.0 μm) and quantified using mass spectrometry. The mobile phase was a mixture of water with 0.1% formic acid and acetonitrile with 0.1% formic acid (91:9). The standard curve ranged from 1 to 500 ng/mL. Intra- and Inter-assay variability for hydromorphone was10%, and the average recovery was90%. The LLOQ was 1 ng/mL. This method was successfully applied to the analysis of hydromorphone samples at this institution. This method could be useful to those investigators dealing with small sample volumes, particularly when conducting pharmacokinetic studies that require multiple sampling from the same animal. |
| Databáze: | OpenAIRE |
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