Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men
| Autor: | Katrin A. Kopf-Bolanz, Barbara Walther, Magali Chollet, Kurt Laederach, Charlotte Soneson, Ueli Bütikofer, Nadine Zangger, Mauro Delorenzi, Doreen Gille, Flurina Schwander, Guy Vergères |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty medicine.medical_treatment Endocrinology Diabetes and Metabolism Clinical Biochemistry 610 Medicine & health Biology Diet High-Fat Biochemistry Blood cell Transcriptome 03 medical and health sciences Internal medicine medicine Ingestion Humans Obesity Dose–Response Gene expression High-fat meal Insulin Oxidative phosphorylation Molecular Biology Meal Nutrition and Dietetics 630 Agriculture digestive oral and skin physiology Middle Aged medicine.disease Postprandial Period Crossover study Lipids 030104 developmental biology Postprandial medicine.anatomical_structure Endocrinology Gene Expression Regulation Multivariate Analysis Energy Intake |
| Zdroj: | Gille, Doreen; Zangger, Nadine; Soneson, Charlotte; Bütikofer, Ueli; Delorenzi, Mauro; Schwander, Flurina; Kopf-Bolanz, Katrin A; Chollet, Magali; Walther, Barbara; Laederach, Kurt; Vergères, Guy (2017). Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men. The journal of nutritional biochemistry, 43, pp. 156-165. Elsevier 10.1016/j.jnutbio.2017.02.012 The Journal of Nutritional Biochemistry, vol. 43, pp. 156-165 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2017.02.012 |
| Popis: | We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to investigate differentially expressed genes in both study groups. We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response indicative of a loss of metabolic flexibility. The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may represent a sensitive way to evaluate the qualitative impact of food on human health. |
| Databáze: | OpenAIRE |
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