Survival of Transplanted Rat Bone Marrow-Derived Osteogenic Stem Cells In Vivo
| Autor: | Matthias Gierloff, Joerg Wiltfang, Hendrik Terheyden, Yahya Açil, Jürgen Hedderich, Corinna E. Zimmermann |
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| Rok vydání: | 2011 |
| Předmět: |
Calcium Phosphates
Male Pathology medicine.medical_specialty Stromal cell Biomedical Engineering H&E stain Biocompatible Materials Bone Marrow Cells Bioengineering Rat Bone Marrow Biochemistry Biomaterials Osteogenesis In vivo medicine Animals Bone Marrow Transplantation Tissue Engineering Chemistry CD68 Stem Cells Histology Anatomy Rats Durapatite Bone Substitutes Subcutaneous implantation Stromal Cells Stem cell |
| Zdroj: | Tissue Engineering Part A. 17:1147-1156 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2009.0577 |
| Popis: | This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann® BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (n = 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (n = 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4',6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (p 0.001). Fourteen days postimplantation MSC could no longer be identified. Additionally, starting on day 3 postimplantation, cellular disintegration was noted. Multinucleated giant cells were present in constructs and controls on day 7 and increased to day 14 postimplantation. These results indicate that ectopically transplanted MSC survive for a rather short time after implantation. Possible reasons for early cell death are discussed. |
| Databáze: | OpenAIRE |
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