Presence of a reversible inhibitor for human lymphoid cell DNA synthesis in the extracts of human peripheral lymphocytes
| Autor: | Photini S. Papageorgiou, Lance M. Tibbetts, Philip R. Glade |
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| Rok vydání: | 1975 |
| Předmět: |
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Lymphocyte Immunology Cell Endogeny Cell Separation Biology Lymphocyte Activation Tritium Cell Line HeLa Lectins medicine Humans Trypsin Carbon Radioisotopes Lymphocytes Thermolabile Lung Cells Cultured Deoxyribonucleases DNA synthesis Tissue Extracts DNA Hydrogen-Ion Concentration Embryo Mammalian biology.organism_classification Molecular biology medicine.anatomical_structure Cell culture Lymphocyte Culture Test Mixed HeLa Cells Thymidine medicine.drug |
| Zdroj: | Cellular Immunology. 18:384-395 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(75)90067-2 |
| Popis: | An endogenous inhibitor of human lymphocyte DNA synthesis contained in extracts of purified human peripheral lymphocytes is described. It was found that the peripheral lymphocyte extract inhibits the DNA synthesis of phytohemagglutinin (PHA) stimulated human peripheral lymphocytes, lymphocytes in mixed lymphocyte culture, and human lymphoid cells in a long-term culture (PGLC-33H). This extract did not inhibit the DNA synthesis of nonlymphoid cells including HeLa and human embryonic lung. The effects of the inhibitor were reversible and noncytotoxic. Initial characterization showed the inhibitor to be thermolabile, DNase resistant, trypsin sensitive, and stable in a pH range 5.4–8.4. It appears that the inhibitor contained in the purified human peripheral lymphocyte extract is similar to a previously described inhibitor extracted from a human lymphoid cell line (PGLC-33H). Quantitation of the inhibitor in various lymphoid cell populations showed the amount of inhibitor per cell to be higher in resting peripheral lymphocytes than in PHA stimulated peripheral lymphocytes or human lymphoid cells in long-term culture (PGLC-33H). This data suggest that the inhibitor described may play a regulatory role in lymphocyte metabolism. |
| Databáze: | OpenAIRE |
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