Activity of synthetic chalcones in hamsters experimentally infected with Leishmania (Viannia) braziliensis
| Autor: | Thaís Gomes Verzignassi Silveira, Sandra Mara Alessi Aristides, Maria Valdrinez Campana Lonardoni, Evandra Maria Voltarelli, Bruna Muller Cardoso, Luzmarina Hernandes, Sara Negrão Lopes, Tatiane França Perles de Mello, Heriberto Rodrigues Bitencourt |
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| Rok vydání: | 2015 |
| Předmět: |
Chalcone
Pathology medicine.medical_specialty Antiprotozoal Agents Leishmaniasis Cutaneous Spleen Biology Pharmacology Leishmania braziliensis Parasite Load chemistry.chemical_compound Chalcones Cutaneous leishmaniasis In vivo Cricetinae medicine Animals General Veterinary Histology General Medicine biology.organism_classification Leishmania medicine.disease Infectious Diseases medicine.anatomical_structure Liver chemistry Insect Science Toxicity Female Parasitology |
| Zdroj: | Parasitology Research. 114:3587-3600 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-015-4581-1 |
| Popis: | The purpose of this study is to evaluate the toxicity of synthetic chalcones 1 and 2 in uninfected hamsters and anti-Leishmania activity of synthetic chalcones 1 and 2 in hamsters infected with Leishmania (Viannia) braziliensis. For the toxicity test, uninfected animals were treated with chalcones 1 and 2, and clinical and biochemical parameters and histological aspects of the liver and kidneys were assessed. Chalcones 1 and 2 were then intraperitoneally or topically administered (10 mg/kg body weight) three times per week in animals infected with promastigotes of L. (V.) braziliensis. We monitored the thickness of the infected footpads, determined parasitic load, performed histological analysis, and detected apoptosis in situ. The results were analyzed using Student’s t test and Mann-Whitney test at a significance level of 5 %. Neither of the chalcones showed toxicity. Chalcone 2 administered intraperitoneally significantly reduced the thickness of the infected footpad compared with the beginning of treatment. The parasite load of the lymph node and spleen was reduced in the groups treated with chalcones 1 (topical) and 2 (intraperitoneal). Chalcone 2 (topical) reduced parasite burden only in the lymph node. The histological analysis revealed reconstitution of the tissue and reductions of inflammation and apoptosis in the infected footpad in these groups. The synthetic chalcones 1 (topical) and 2 (intraperitoneal and topical) at a dose of 10 mg/kg showed anti-Leishmania activity in vivo, no renal or hepatic toxicity, and a reduction of apoptosis of the cells in the lesions. These chalcones may have substantial potential for the treatment of cutaneous leishmaniasis. |
| Databáze: | OpenAIRE |
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