Ex vivo approach supports both direct and indirect actions of melatonin on immunity in pike-perch Sander lucioperca
| Autor: | Sébastien Baekelandt, Mickaël Dubois, Syaghalirwa N.M. Mandiki, Patrick Kestemont, Valérie Cornet, Jérôme Lambert |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Adaptive Immunity Aquatic Science Biology Head Kidney/drug effects Cortisol Melatonin 03 medical and health sciences Immune system Immunity Internal medicine Immunity Innate/drug effects Gene expression medicine Animals Environmental Chemistry Melatonin/administration & dosage Receptor Pike-perch Adaptive Immunity/drug effects 04 agricultural and veterinary sciences General Medicine Head Kidney Acquired immune system Immunity Innate Perches/immunology Innate/drug effects 030104 developmental biology Endocrinology Perches Spleen/drug effects 040102 fisheries 0401 agriculture forestry and fisheries Spleen hormones hormone substitutes and hormone antagonists Ex vivo Hormone medicine.drug |
| Zdroj: | Baekelandt, S, Cornet, V, Mandiki, R, Lambert, J, Dubois, M & Kestemont, P 2021, ' Ex vivo approach supports both direct and indirect actions of melatonin on immunity in pike-perch Sander lucioperca ', Fish and Shellfish Immunology, vol. 112, pp. 143-150 . https://doi.org/10.1016/j.fsi.2021.03.005 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2021.03.005 |
| Popis: | The melatonin hormone, which is a multifunctional molecule in vertebrates, has been shown to exert complex actions on the immune system of mammals. In teleosts, the immunomodulatory capacity of this hormone has seldom been investigated. In the present experiment, we exposed ex vivo spleen and head kidney tissues of pike-perch to melatonin (Mel) and cortisol (Cort). We applied three concentrations of both hormones, alone and in combination, namely (1) Mel (10, 100 or 1000 pg mL−1) (2) Cort (50, 500 or 5000 ng mL−1) (3) Mel + Cort (10 + 50, 100 + 500 or 1000 pg mL−1+5000 ng mL−1). Pure medium without Mel or Cort served as control. After 15 h of incubation, we assessed the expression of a set of immunity-related genes, including genes encoding for pro-inflammatory proteins (il-1β, cxcl8 and tnf-α), acute-phase proteins (fgl2, fth1, hepc, hp and saa1) and key factors of the adaptive immune system (fκbp4 and tcrg). Both Mel and Cort, when used alone or combined at physiological concentrations, significantly influenced immune gene expressions that may lead to a global immune stimulation. Our results support both, an indirect action of the Mel hormone on the immune system through the regulation of intermediates such as Cort, as well as a direct action on immune targets through specific receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect