Cerebral endothelial cell culture. I. The presence of beta 2 and alpha 2-adrenergic receptors linked to adenylate cyclase activity
| Autor: | Maria Spatz, Irina L. Karnushina, Joliet Bembry |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
Endothelium Adrenergic receptor Epinephrine Vasoactive intestinal peptide Bradykinin Biology Cyclase General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Norepinephrine Internal medicine Receptors Adrenergic beta medicine Animals General Pharmacology Toxicology and Pharmaceutics Cells Cultured Isoproterenol Brain General Medicine Receptors Adrenergic alpha Adenosine Rats Receptors Adrenergic Kinetics Endocrinology medicine.anatomical_structure chemistry Animals Newborn Cyclase activity Acetylcholine medicine.drug Adenylyl Cyclases |
| Zdroj: | Life sciences. 30(10) |
| ISSN: | 0024-3205 |
| Popis: | Cultured endothelial cells derived from cerebral microvessels separated from 2-day-old rat brain contain a specific beta 2 and alpha 2-adrenergic sensitive adenylate cyclase (AC). Among the various tested hormones, PGE1 and PGE2 were found to be the most potent activators, while adenosine, angiotensin I and II, gamma-aminobutyric acid and vasoactive intestinal peptide inhibited the enzyme activity. However, acetylcholine, histamine, serotonin, glycine, glutamine, bradykinin, neurotensin and vasopressin (Lysine and Arginine) had no effect on the adenylate cyclase activity in this model. The susceptibility of the cerebrovascular endothelial AC system to the vasoactive substances as well as presence of beta 2 and alpha 2-type adrenergic receptors in the cultured endothelium provides additional support for the proposed endothelial involvement in the regulation of cerebrovascular permeability and blood flow. |
| Databáze: | OpenAIRE |
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