Phenotypic and Molecular Characterization of β-Lactamases among Enterobacterial Uropathogens in Southeastern Nigeria

Autor:	I. R. Iroha, Beri K, Masood A. Shariff, Malachy C Ugwu, Ugochukwu Moses Okezie, C.M. Nnajide, Charles Okechukwu Esimone
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Microbiology (medical) Salmonella Article Subject medicine.drug_class β lactamases Antibiotics Drug resistance Infectious and parasitic diseases RC109-216 Biology biochemical phenomena metabolism and nutrition medicine.disease_cause bacterial infections and mycoses Phenotype Microbiology QR1-502 Southeastern Nigeria Infectious Diseases Antibiotic resistance medicine polycyclic compounds bacteria Gene Research Article
Zdroj:	The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien des Maladies Infectieuses et de la Microbiologie Médicale Canadian Journal of Infectious Diseases and Medical Microbiology, Vol 2020 (2020)
ISSN:	1712-9532
DOI:	10.1155/2020/5843904
Popis:	Little is known about the molecular basis of antibiotic resistance among uropathogens in Southeast Nigeria. The aim of the study was to characterize enterobacterial uropathogens with respect to drug resistance. One hundred (100) enterobacterial uropathogens were studied. Their antibiotic susceptibility patterns were evaluated using disk diffusion, screened, and confirmed phenotypically for the presence ofβ-lactamases: ESBL, AmpC, carbapenemase, and MBLs. Screen positives were further tested for variousβ-lactamase genes by PCR. Our isolates showed variable resistance to most drugs tested. Out of the 58 ESBL screen positiveE. coli,35 were confirmed positive with PCR. The predominant ESBL gene was blaTEMwhile blaSPMwas the most prevalent among MBL genes. Forty-six percentage of the screen positiveSalmonellaisolates coharbored blaTEM + SHVgenes. Nine of the 10 ESBL screen positiveK. pneumoniaewere phenotypically and PCR positive. Three isolates ofK. pneumoniaewere positive for MBL genes. All the 10 C. freundiiwere positive for ESBL genes. The study showed high prevalence of drug-resistant genes among the enterobacterial uropathogens. Majority of the uropathogens harbored >1 antibiotic-resistant gene, and the most predominant gene was ESBL (blaTEM) followed by the MBL (SPM) gene.
Databáze:	OpenAIRE
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