Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury
| Autor: | Igor Pantic, Zagorka Vitic, Rada Jeremic, Sanja Dacic, Senka Pantic, Claude Brodski, Sanja Pekovic, Marina Djelic, Predrag Brkic |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Photomicrography Traumatic brain injury Gray-level co-occurrence matrix analysis Neuropathology Hippocampal formation Hippocampus 03 medical and health sciences 0302 clinical medicine medicine Animals Dentate gyrus Sensorimotor cortex ablation Texture Rats Wistar Instrumentation 030304 developmental biology Neurons 0303 health sciences business.industry Granule (cell biology) Cognition medicine.disease Granule cell Gray level Disease Models Animal medicine.anatomical_structure nervous system Brain Injuries Dentate Gyrus business Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Microscopy and Microanalysis |
| Popis: | Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|