A peripheral benzodiazepine receptor targeted agent for in vitro imaging and screening
| Autor: | Nephi Stella, H. Charles Manning, Kristin B. Cederquist, Darryl J. Bornhop, Sarah Haviland, Sarah M. Smith, Mingfeng Bai, Michelle Sexton |
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| Rok vydání: | 2006 |
| Předmět: |
Fluorophore
medicine.drug_class Biomedical Engineering Pharmaceutical Science Bioengineering Mitochondrion Cell Line chemistry.chemical_compound Receptors GABA medicine Animals Humans Receptor Pharmacology Benzodiazepine Molecular Structure Chemistry Organic Chemistry Isoquinolines Fluorescence In vitro Mitochondria Rats Succinate Dehydrogenase Biochemistry Cell culture Molecular imaging Biotechnology |
| Zdroj: | Bioconjugate chemistry. 17(3) |
| ISSN: | 1043-1802 |
| Popis: | We developed a molecular imaging agent (MIA), a conjugable form of PK11195 (conPK11195) coupled to a lissamine dye (Liss-ConPK11195), which targets the peripheral benzodiazepine receptor (PBR). To determine that our compound specifically binds to this 18 kDa protein, primarily expressed on the mitochondria, we performed classic binding studies on live MDA-MB-231 breast cancer cells and measured fluorescence in cell fractions of C6 glioma cells. We found that conPK11195 conjugated to the fluorophore retained significant binding to its target. Here we demonstrate the utility of the agent for in vitro imaging of live cells by specific binding to the protein of interest. |
| Databáze: | OpenAIRE |
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