Injectable hybrid delivery system composed of gellan gum, nanoparticles and gentamicin for the localized treatment of bone infections

Autor: Anna Drożdż, Wojciech Chrzanowski, Elżbieta Pamuła, Monika Brzychczy-Włoch, Małgorzata K. Włodarczyk-Biegun, Piotr Dobrzyński, Małgorzata Krok-Borkowicz, Urszula Posadowska
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Staphylococcus aureus
medicine.medical_specialty
Materials science
poly(lactide-co-glycolide) (PLGA)
0206 medical engineering
Pharmaceutical Science
Nanoparticle
biocompatible materials
Microbial Sensitivity Tests
02 engineering and technology
Site specificity
Polysaccharide
Bone Infection
chemistry.chemical_compound
Polylactic Acid-Polyglycolic Acid Copolymer
Staphylococcus epidermidis
medicine
Humans
gentamicin (GENT)
Lactic Acid
Controlled drug delivery
chemistry.chemical_classification
Drug Carriers
Polysaccharides
Bacterial
osteomyelitis
021001 nanoscience & nanotechnology
020601 biomedical engineering
Gellan gum
Anti-Bacterial Agents
Surgery
BBP Bioconversion
chemistry
Injections
Intravenous
Gentamicin
nanoparticles
Delivery system
Gentamicins
0210 nano-technology
Drug carrier
Physical Chemistry and Soft Matter
Polyglycolic Acid
Biomedical engineering
medicine.drug
gellan gum
Zdroj: Expert Opinion on Drug Delivery 13 (2016) 5
Expert Opinion on Drug Delivery, 13(5), 613-620
ISSN: 1742-5247
Popis: Objectives: Bone infections are treated with antibiotics administered intravenously, antibiotic-releasing bone cements or collagen sponges placed directly in the infected area. These approaches render limited effectiveness due to the lack of site specificity and invasiveness of implanting cements and sponges. To address these limitations, we developed a novel polysaccharide hydrogel-based injectable system that enables controlled delivery of gentamicin (GENT). Its advantages are minimal invasiveness, and localized and finely regulated release of the drug. Methods: GENT was incorporated both directly within the gellan gum hydrogel and into poly(L-lactide-co-glycolide) nanoparticles embedded into the hydrogel. Results: We confirmed the injectability of the system and measured extrusion force was 15.6 ± 1.0 N, which is suitable for injections. The system set properly after the injection as shown by rheological measurements. Desired burst release of the drug was observed within the first 12 h and the dose reached ~27% of total GENT. Subsequently, GENT was released gradually and sustainably: ~60% of initial dose within 90 days. In vitro studies confirmed antimicrobial activity of the system against Staphylococcus spp. and cytocompatibility with osteoblast-like cells. Conclusions: Developed injectable system enables minimally invasive, local and sustained delivery of the pharmaceutically relevant doses of GENT to combat bone infections.
Databáze: OpenAIRE
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