Calcium-Enriched Nanofibrillated Cellulose/Poloxamer in-situ Forming Hydrogel Scaffolds as a Controlled Delivery System of Raloxifene HCl for Bone Engineering
| Autor: | Rabab Kamel, Nermeen A. Elkasabgy, Nahla A. El-Wakil |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
Biophysics Pharmaceutical Science chemistry.chemical_element Bioengineering Poloxamer Calcium calcium phosphate Biomaterials chemistry.chemical_compound bone regeneration International Journal of Nanomedicine Spectroscopy Fourier Transform Infrared Drug Discovery medicine Cellulose Bone regeneration Original Research Tissue Engineering Tissue Scaffolds Raloxifene Hydrochloride Organic Chemistry raloxifene hydrochloride in-situ forming hydrogel scaffolds Hydrogels General Medicine Retraction Chemical engineering chemistry Poloxamer 407 Self-healing hydrogels nanofibrillated cellulose medicine.drug |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Purpose TEMPO-oxidized nanofibrillated cellulose (TONFC) originating from an agricultural waste (sugar cane) was utilized to prepare injectable in-situ forming hydrogel scaffolds (IHS) for regenerative medicine. Methods TONFC was prepared and characterized for its morphology and chemical structure using TEM and FT-IR, respectively. The cold method was applied to prepare hydrogels. Various concentrations of poloxamer 407 were added to the prepared TONFC (0.5%w/w). Different sources of calcium, Fujicalin® (DCP) or hydroxyapatite (TCP), were used to formulate the aimed calcium-enriched raloxifene hydrochloride-loaded IHS. Gelation temperature, drug content, injectability and in-vitro drug release were evaluated along with the morphological characters. Cytocompatibility studies and tissue regeneration properties were assessed on Saos-2 cells. Results TEM photograph of TONFC showed fibrous nanostructure. The selected formulation “Ca-IHS4” composed of TONFC+15% P407+10% TCP showed the most prolonged release pattern for 12 days with the least burst effect (about 25% within 24 h). SEM micro-photographs of the in-situ formed scaffolds showed a highly porous 3D structure. Cytocompatibility studies of formulation “Ca-IHS4” revealed the biocompatibility as well as improved cell adhesion, alkaline phosphatase enzyme activity and calcium ion deposition. Conclusion The outcomes suggest that Ca-IHS4 presents a simple, safe-line and non-invasive strategy for bone regeneration. Graphical Abstract |
| Databáze: | OpenAIRE |
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