Monitoring of cyclic GMP during cerebellar microdialysis in freely-moving rats as an index of nitric oxide synthase activity
| Autor: | Maurizio Raiteri, F. Vallebuona |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Microdialysis N-Methylaspartate Arginine Phosphodiesterase Inhibitors Motor Activity Receptors N-Methyl-D-Aspartate Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Internal medicine 1-Methyl-3-isobutylxanthine Cerebellum medicine Animals Channel blocker Phosphodiesterase inhibitor Cyclic GMP Monitoring Physiologic biology General Neuroscience Rats Dizocilpine Nitric oxide synthase Endocrinology chemistry biology.protein NMDA receptor Amino Acid Oxidoreductases Nitric Oxide Synthase Extracellular Space medicine.drug |
| Zdroj: | Neuroscience. 57(3) |
| ISSN: | 0306-4522 |
| Popis: | The nitric oxide synthase/cyclic GMP pathway has been studied in vivo in the adult rat cerebellum by monitoring the levels of extracellular cyclic GMP during microdialysis in conscious unrestrained animals. The basal cyclic GMP efflux was concentration-dependently reduced upon local infusion of the nitric oxide synthase inhibitor N G -nitro- l -arginine (10 μM−1 mM). The nitric oxide donor S-nitroso-N-penicillamine , perfused through the dialysis probe at 1 mM, increased by about 200% the extracellular levels of cyclic GMP. The glutamate receptor agonist N-methyl- d -aspartate (500μM) produced a cyclic GMP response which was abolished by the selective receptor antagonist d -2-amino-5-phosphonovaleric acid (500 μM) or by N G -nitro- l -arginine (10 μM). The elevation of cyclic GMP levels caused by local infusion of 500 μM N-methyl- d -aspartate was also abolished by parentheral administration of the N-methyl- d -aspartate channel blocker dizocilpine (0.4 mg/kg, i.p.). Local perfusion of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (1 mM) increased by about 150% the extracellular levels of cyclic GMP. It is concluded that cyclic GMP collected during in vivo microdialysis reflects nitric oxide synthase activity in the rat cerebellum. The technique may be utilized to investigate the pathophysiology and the pharmacology of the nitric oxide/cyclic GMP pathway in the cerebellum of living animals. |
| Databáze: | OpenAIRE |
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