Alcohol administration during adulthood induces alterations of parvalbumin and glial fibrillary acidic protein immunoreactivity in rat hippocampus and cingulate cortex
| Autor: | Ekkasit Kumarsit, Surapong Vongvatcharanon, Wandee Udomuksorn, Sirirak Mukem, Uraporn Vongvatcharanon |
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| Rok vydání: | 2009 |
| Předmět: |
Cingulate cortex
Male medicine.medical_specialty Histology Alcohol Drinking Hippocampus Alcohol Gyrus Cinguli chemistry.chemical_compound Internal medicine Glial Fibrillary Acidic Protein medicine Animals Rats Wistar Neurons Ethanol biology Glial fibrillary acidic protein Central Nervous System Depressants Cell Biology General Medicine Anatomy Immunohistochemistry Rats Endocrinology Parvalbumins nervous system chemistry Astrocytes biology.protein GABAergic Parvalbumin |
| Zdroj: | Acta histochemica. 112(4) |
| ISSN: | 1618-0372 |
| Popis: | Alcohol induces impairment of cognition, learning and memory. Neurotoxic effects of alcohol on the pathology of the hippocampus and the cingulate cortex were investigated in experimental rats. Parvalbumin (PV), a calcium-binding protein, is a crucial component of GABAergic neurons and glial fibrillary acidic protein immunoreactive (GFAP-ir) astrocytes have been used as markers. We investigated the effects of ethanol exposure during adulthood on the PV-ir neurons and GFAP-ir astrocytes in the hippocampus and the cingulate cortex of 3-month-old male Wistar rats. The rats were divided into 2 groups: control (C) and alcohol-exposed groups. The control group received distilled water whereas the alcohol-exposed groups received either a low dose (20%w/v, LD) or high dose (40%w/v, HD) of ethanol for periods of 21 days, 3 or 6 months. The brains of the animals were processed for immunohistochemistry using anti-parvalbumin and anti-GFAP antibodies and the numbers of PV immunoreactive (PV-ir) neurons and GFAP-ir astrocytes were counted/unit area. For each period of administration, the number of PV-ir neurons was significantly reduced for groups exposed to both the low and the high doses of ethanol compared to those of control groups in both the hippocampus and the cingulate cortex (p |
| Databáze: | OpenAIRE |
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