In vitro lesion repair by human amnion epithelial and mesenchymal cells
| Autor: | Roland Zimmermann, Grozdana Bilic, Nicole Ochsenbein-Kölble, Heike Hall, Renate Huch |
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| Rok vydání: | 2004 |
| Předmět: |
Cell type
Pathology medicine.medical_specialty Time Factors medicine.medical_treatment Placenta Gestational Age Lesion Mesoderm Fetal membrane Medicine Humans Amnion Cells Cultured Platelet-Derived Growth Factor Rupture Wound Healing business.industry Tumor Necrosis Factor-alpha Growth factor Mesenchymal stem cell Obstetrics and Gynecology Fibrinogen Epithelial Cells medicine.anatomical_structure embryonic structures Immunology Tetradecanoylphorbol Acetate Tumor necrosis factor alpha Female medicine.symptom business Wound healing Cell Division |
| Zdroj: | American journal of obstetrics and gynecology. 190(1) |
| ISSN: | 0002-9378 |
| Popis: | Objective The purpose of this study was to compare wound healing by human amnion epithelial and mesenchymal cells from preterm and term placenta with the use of an in vitro lesion repair assay. Study design Lesions were created in confluent monolayers of amnion epithelial and mesenchymal cells from preterm and term placentas. The repair was monitored by the measurement of the lesion area and the response to potential stimulants (platelet-derived growth factor, tumor necrosis factor-α, fibrinogen, and phorbol myristate acetate). Cell proliferation was detected with 5-bromodeoxyuridine staining. Results Lesion repair was complete within 40 hours in control epithelial cultures from preterm and term placenta but incomplete in mesenchymal cultures (preterm cells, 80%; term cells, 40%). Platelet-derived growth factor, tumor necrosis factor-α, fibrinogen, and phorbol myristate acetate did not accelerate repair in either cell type. Conclusion An in vitro lesion repair assay revealed the differences in lesion repair capacity between amnion epithelial and mesenchymal cells and between mesenchymal cells from preterm and term placenta. |
| Databáze: | OpenAIRE |
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