T-2 toxin induces cytotoxicity and disrupts tight junction barrier in SerW3 cells
| Autor: | Güldeniz Selmanoğlu, Elif Karacaoğlu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cell Survival Health Toxicology and Mutagenesis Cell Biology Toxicology medicine.disease_cause Occludin Cell Line Tight Junctions 03 medical and health sciences chemistry.chemical_compound Electric Impedance medicine Animals Cytotoxic T cell Viability assay Cytotoxicity Pharmacology Sertoli Cells Dose-Response Relationship Drug L-Lactate Dehydrogenase Tight junction Toxin General Medicine Rats Cell biology T-2 Toxin 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation chemistry Trypan blue |
| Zdroj: | Environmental Toxicology and Pharmacology. 56:259-267 |
| ISSN: | 1382-6689 |
| DOI: | 10.1016/j.etap.2017.10.005 |
| Popis: | T-2 toxin, which is produced in grain and grain products as a secondary metabolite by Fusarium species, is also potentially dangerous for human health. Up to date, no study was reported the cytotoxicity of T-2 toxin on SerW3 cells in the perspective of junctional barriers. This study focused on revealing the cytotoxic effects of T-2 on Sertoli cells associated with cell junctional barriers. In the present study, SerW3 cells were exposed to T-2 toxin at 12, 120 and 1200 ng/ml doses for 24 and 48 h. Cytotoxicity tests including cell viability (MTT), lactate dehydrogenase (LDH) cytotoxicity test and trypan blue exclusion assay were performed. Occludin, ZO-1, N-cadherin and beta-catenin were immunolabelled, expressions of occludin and N-cadherin were determined by western blotting. SerW3 cell barrier integrity was measured by transepithelial electrical resistance (TEER). Cytotoxicity caused by T-2 toxin increased in a dose dependent manner, expressions of proteins and TEER measurement decreased. This study may underlie the early targets of T-2 toxin on SerW3 cells mimicking blood testis barrier in vitro. |
| Databáze: | OpenAIRE |
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