Target Gene Sequencing To Define the Susceptibility of Neisseria meningitidis to Ciprofloxacin
| Autor: | Raquel Abad, Julio A. Vázquez, Rocío Enríquez, Muhamed-Kheir Taha, Eva Hong, Paola Stefanelli, Cecilia Fazio, Keith A. Jolley, Frédéric J. Veyrier, Sara Thulin Hedberg, Magnus Unemo, Ala-Eddine Deghmane |
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| Přispěvatelé: | Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae (CNR), Institut Pasteur [Paris] (IP), Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Instituto de Salud Carlos III [Madrid] (ISC), Department of Infectious, Parasitic and Immune-Mediated Diseases [Rome], Istituto Superiore di Sanità (ISS), University of Oxford, This work was supported by the Institut Pasteur and the Institut de Veille Sanitaire (France) and by grants from the Örebro County Council Research Committee and the Foundation for Medical Research at Örebro University Hospital, Örebro, Sweden., Institut Pasteur [Paris], Istituto Superiore di Sanita [Rome], University of Oxford [Oxford] |
| Rok vydání: | 2013 |
| Předmět: |
MESH: Mice
Transgenic Gyra gene Mice Transgenic Microbial Sensitivity Tests Neisseria meningitidis Biology MESH: Meningococcal Infections medicine.disease_cause DNA gyrase MESH: Neisseria meningitidis Microbiology Mice 03 medical and health sciences Ciprofloxacin resistance Data sequences Anti-Infective Agents [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Ciprofloxacin Mechanisms of Resistance MESH: Anti-Bacterial Agents medicine Animals MESH: Animals Pharmacology (medical) MESH: Ciprofloxacin MESH: Mice 030304 developmental biology Pharmacology MESH: Microbial Sensitivity Tests 0303 health sciences 030306 microbiology Breakpoint biochemical phenomena metabolism and nutrition bacterial infections and mycoses Virology Anti-Bacterial Agents 3. Good health Meningococcal Infections Infectious Diseases DNA Gyrase Female Target gene MESH: Female MESH: DNA Gyrase medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy, 2013, 57 (4), pp.1961-1964. ⟨10.1128/AAC.02184-12⟩ Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2013, 57 (4), pp.1961-1964. ⟨10.1128/AAC.02184-12⟩ |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.02184-12 |
| Popis: | Meningococcal gyrA gene sequence data, MICs, and mouse infection were used to define the ciprofloxacin breakpoint for Neisseria meningitidis . Residue T91 or D95 of GyrA was altered in all meningococcal isolates with MICs of ≥0.064 μg/ml but not among isolates with MICs of ≤0.032 μg/ml. Experimental infection of ciprofloxacin-treated mice showed slower bacterial clearance when GyrA was altered. These data suggest a MIC of ≥0.064 μg/ml as the ciprofloxacin breakpoint for meningococci and argue for the molecular detection of ciprofloxacin resistance. |
| Databáze: | OpenAIRE |
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