Cytokines and Effector/Regulatory Cells Characterization in the Physiopathology of Cutaneous Lupus Erythematous: A Cross-Sectional Study
| Autor: | Ana Bety Enríquez, Yeraldin Esquivel, Janette Furuzawa-Carballeda, David Faz-Muñoz, Carlos Pacheco-Molina, Gabriela Hernández-Molina, Silvia Méndez-Flores |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Pathology medicine.medical_specialty Article Subject Discoid lupus erythematosus Adolescent Immunology T-Lymphocytes Regulatory Subacute cutaneous lupus erythematosus 03 medical and health sciences Interferon-gamma Young Adult lcsh:Pathology medicine Lupus Erythematosus Cutaneous Humans IL-2 receptor Aged Lupus erythematosus Systemic lupus erythematosus business.industry Interleukins Interleukin-17 FOXP3 hemic and immune systems Cell Biology Middle Aged medicine.disease Interleukin-10 Interleukin 10 030104 developmental biology Cross-Sectional Studies Interleukin 17 Interleukin-4 business lcsh:RB1-214 Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2016 (2016) |
| ISSN: | 1466-1861 |
| Popis: | We compared the presence of diverse cytokines and regulatory T and B cells in skin biopsies of discoid lupus erythematosus (DLE) and subacute cutaneous lupus erythematosus (SCLE). We included 19 patients with DLE, 13 with SCLE, 8 healthy controls, and 5 patients with hypertrophic scars. We assessed the CLASI activity score. To determine IL-22-producing cells and the subpopulation of CD4+/IL-17A+-, CD4+/IL-4+-, and CD4+/IFN-γ+-expressing T cells, CD123+/IDO+pDCs, CD25+/Foxp3+Tregs, and CD20+/IL-10+-producing B cells, an immunostaining procedure was performed. Also intracellular IL-22, IL-17, IL-4, IFN-γ, and Foxp3 in CD4 T cells, IL-10 in B cells, and IDO in pDCs were analyzed by flow cytometry in peripheral blood. The main cellular participation in both lupus groups was IL-17- and IL-22-producing cell responses both at skin and at peripheral blood but prevailed in DLE. The CLASI activity scores negatively correlated with Th22 subpopulation and positively correlated with CD25+/Foxp3+Treg cells. In conclusion a proinflammatory and regulatory imbalance coexists in cutaneous lupus, both responses being more intense in DLE. |
| Databáze: | OpenAIRE |
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