An improved definition of mouse mammary epithelial side population cells
| Autor: | I. Titley, Matthew J. Smalley, Alan Ashworth |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty ATP Binding Cassette Transporter Subfamily B Indoles Time Factors Cell Survival Immunology Population Cell Mammary cells Biology Side-Population Cell Mice Mammary Glands Animal Side population medicine ATP Binding Cassette Transporter Subfamily G Member 2 Animals Immunology and Allergy education Genetics (clinical) Fluorescent Dyes Transplantation education.field_of_study Stem Cells Epithelial Cells Cell Biology Flow Cytometry Cell biology Staining medicine.anatomical_structure Verapamil Oncology ATP-Binding Cassette Transporters Benzimidazoles Female Stem cell |
| Zdroj: | Cytotherapy. 7:497-508 |
| ISSN: | 1465-3249 |
| DOI: | 10.1080/14653240500361145 |
| Popis: | Background Mammary epithelial side population cells have been suggested as candidate mammary stem cells. To date, for technical reasons, these cells have been poorly defined and cross-comparison of data between different laboratories has been difficult. Here, we set out to define mammary side population cells in a way that improves the ability to carry out such comparisons. Methods Mouse mammary epithelial cells were stained with Hoechst 33342. Light scatter, PI staining and clonogenicity of different regions of the Hoechst profile were examined. Time–course analyzes of Hoechst 33342 loading were carried out. Results Detailed examination of the light scatter and PI staining of Hoechst 33342-stained mammary cells enabled single live side population and non-side population cells to be defined with greater accuracy. Comparison of ABC pump inhibitors identified potential discrepancies in results obtained using these inhibitors. Time–course analyzes enabled side populations cells to be identified as a dynamic cell population that could be defined accurately by using the relationship between Hoechst 33342-staining profiles of consecutive time points. Discussion Defining the side population of solid tissues as a ‘stabilized side population percentage’ will enable a more rigorous study of the side population phenomenon and improve evaluation of results from different laboratories. |
| Databáze: | OpenAIRE |
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