Identification of the agent causing visceral leishmaniasis in Uzbeki and Tajiki foci by analysing parasite DNA extracted from patients' Giemsa-stained tissue preparations
| Autor: | Mohammad Zahangir Alam, Shavkat A Razakov, Fatullaeva Aa, Ponomareva Vi, Katrin Kuhls, Nasyrova Rm, Lionel F. Schnur, Kovalenko Da, Gabriele Schönian |
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| Rok vydání: | 2009 |
| Předmět: |
Tajikistan
Antibodies Protozoan Giemsa stain parasitic diseases medicine Animals Humans Internal transcribed spacer Leishmania infantum Genetics biology Kinetoplastida Leishmaniasis Uzbekistan biology.organism_classification medicine.disease Leishmania Virology Infectious Diseases Visceral leishmaniasis Microsatellite Leishmaniasis Visceral Animal Science and Zoology Parasitology DNA Intergenic Microsatellite Repeats |
| Zdroj: | Parasitology. 136(9) |
| ISSN: | 1469-8161 |
| Popis: | SUMMARYOur present study is the first attempt to characterizeLeishmaniaparasites from foci in Uzbekistan and Tajikistan endemic for visceral leishmaniasis (VL). PCR-sequencing of the ribosomal internal transcribed spacer 1 and multilocus microsatellite typing (MLMT) were applied to DNA extracted from preparations of Giemsa-stained bone marrow aspirates from 13 cases of VL.L. infantumwas shown to cause VL currently occurring in this area. MLMT applying 14 microsatellite markers, previously shown to be polymorphic for strains of theL. donovanicomplex, revealed that microsatellite profiles of parasites causing human VL in the Namangan and Jizzakh regions in Uzbekistan, and Penjikent region in Tajikistan, basically coincide with those of strains ofL. infantumMON-1. Furthermore, these parasites were assigned to a distinct cluster genetically clearly separated from the populations ofL. infantumMON-1 from Europe, the Middle East and North Africa. The existence of a genetically homogeneous but distinct group ofL. infantumMON-1 indicates that the parasites circulating in the Uzbeki and Tajiki foci studied have been restricted there for a long time rather than having been recently introduced from elsewhere by human or animal reservoir migration. |
| Databáze: | OpenAIRE |
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