Demonstration of a capsule plasmid in Bacillus anthracis
| Autor: | Bruce E. Ivins, C. B. Thorne, Theresa M Koehler, L Battisti, B D Green |
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| Rok vydání: | 1985 |
| Předmět: |
Genotype
Immunology Bacillus thuringiensis Bacillus cereus Virulence Microbiology Plasmid Species Specificity Cell Wall Transduction Genetic Bacteriophages History Ancient Bacillaceae biology Genetic Variation biology.organism_classification Bacillales Bacillus anthracis Infectious Diseases Conjugation Genetic Parasitology Bacteria Plasmids Research Article |
| Zdroj: | Infection and Immunity. 49:291-297 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.49.2.291-297.1985 |
| Popis: | Virulent and certain avirulent strains of Bacillus anthracis harbor a plasmid, designated pXO2, which is involved in the synthesis of capsules. Two classes of rough, noncapsulated (Cap-) variants were isolated from the capsule-producing (Cap+) Pasteur vaccine strains ATCC 6602 and ATCC 4229. One class was cured of pXO2, and the other class still carried it. Reversion to Cap+ was demonstrable only in rough variants which had retained pXO2. Proof that pXO2 is involved in capsule synthesis came from experiments in which the plasmid was transferred by CP-51-mediated transduction and by a mating system in which plasmid transfer is mediated by a Bacillus thuringiensis fertility plasmid, pXO12. Cells of Bacillus cereus and a previously noncapsulated (pXO2-) strain of B. anthracis produced capsules after the acquisition of pXO2. |
| Databáze: | OpenAIRE |
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