Leishmania (L.) amazonensis: Fusion between parasitophorous vacuoles in infected bone-marrow derived mouse macrophages
| Autor: | Michel Rabinovitch, Fernando Real, M. Pouchelet |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Mice
Inbred BALB C biology Macrophages Leishmania mexicana Immunology Leishmaniasis Cutaneous Lipid bilayer fusion Kinetoplastida General Medicine Vacuole biology.organism_classification Leishmania Membrane Fusion Virology Mice Infectious Diseases Multiplicity of infection Recurrence Vacuoles Animals Female Parasitology Cinemicrography Amastigote |
| Zdroj: | Experimental Parasitology. 119:15-23 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2007.12.013 |
| Popis: | [Leishmania(L.)] amazonensis amastigotes reside in macrophages within spacious parasitophorous vacuoles (PVs) which may contain numerous parasites. After sporadic fusion events were detected by time-lapse cinemicrography, PV fusion was examined in two different models. In single infections, it was inferred from the reduction in PV numbers per cell. In a reinfection model, macrophages infected with unlabeled amastigotes were reinfected with GFP-transfected- or carboxyfluorescein diacetate succinimidyl ester-labeled parasites, and fusion was detected by the colocalization of labeled and unlabeled amastigotes in the same PVs. The main findings were: (1) as expected, fusion frequency increased with the multiplicity of infection; (2) most fusion events took place in the first 24h of infection or reinfection, prior to the multiplication of incoming parasites; (3) resident and incoming parasites multiplied at similar rates in fused PVs. The model should be useful in studies of parasite and host cell factors and mechanisms involved in PV fusogenicity. |
| Databáze: | OpenAIRE |
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