NLRP12 is a neutrophil-specific, negative regulator of in vitro cell migration but does not modulate LPS- or infection-induced NF-?B or ERK signalling
| Autor: | Anne Wilson, Kate Schroder, Steffen Schuster, Christina J. Groß, Fabienne Tacchini-Cottier, Kaiwen W. Chen, Alina Zamoshnikova |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine MAPK/ERK pathway Neutrophils Chemokine CXCL1 Immunology Biology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement medicine Animals Immunology and Allergy Leishmania major Mice Knockout Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Innate immune system Macrophages Intracellular Signaling Peptides and Proteins NF-kappa B Pattern recognition receptor Inflammasome NF-κB Cell migration Dendritic Cells Hematology Dendritic cell Cell biology Mice Inbred C57BL 030104 developmental biology Gene Expression Regulation chemistry Organ Specificity Female Signal transduction Signal Transduction 030215 immunology medicine.drug |
| Zdroj: | Immunobiology |
| DOI: | 10.1016/j.imbio.2015.10.001 |
| Popis: | NOD-like receptors (NLR) are a family of cytosolic pattern recognition receptors that include many key drivers of innate immune responses. NLRP12 is an emerging member of the NLR family that is closely related to the well-known inflammasome scaffold, NLRP3. Since its discovery, various functions have been proposed for NLRP12, including the positive regulation of dendritic cell (DC) and neutrophil migration and the inhibition of NF-κB and ERK signalling in DC and macrophages. We show here that NLRP12 is poorly expressed in murine macrophages and DC, but is strongly expressed in neutrophils. Using myeloid cells from WT and Nlrp12(-/)(-) mice, we show that, contrary to previous reports, NLRP12 does not suppress LPS- or infection-induced NF-κB or ERK activation in myeloid cells, and is not required for DC migration in vitro. Surprisingly, we found that Nlrp12 deficiency caused increased rather than decreased neutrophil migration towards the chemokine CXCL1 and the neutrophil parasite Leishmania major, revealing NLRP12 as a negative regulator of directed neutrophil migration under these conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|