DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
| Autor: | Erdem Karatekin, Zhiqun Xi, Farren J. Isaacs, Derek Toomre, John T. Powell, Julien Berro, Nathan D. Williams, Wenjiao Zhou, Chenxiang Lin, Ane Landajuela, Ravikiran Kasula |
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| Rok vydání: | 2020 |
| Předmět: |
Brightness
Fluorescence-lifetime imaging microscopy Quantification methods Microscope Bioengineering Nanotechnology 02 engineering and technology Article law.invention Live cell imaging law Fluorescence microscope DNA origami Animals General Materials Science Fluorescent Dyes chemistry.chemical_classification Mechanical Engineering Biomolecule Proteins General Chemistry DNA 021001 nanoscience & nanotechnology Condensed Matter Physics Fluorescence chemistry Microscopy Fluorescence Quantitative Microscopy 0210 nano-technology |
| Zdroj: | Nano Lett |
| ISSN: | 1530-6992 |
| Popis: | Fluorescence microscopy has been one of the most discovery-rich methods in biology. In the digital age, the discipline is becoming increasingly quantitative. Virtually all biological laboratories have access to fluorescence microscopes, but abilities to quantify biomolecule copy numbers are limited by the complexity and sophistication associated with current quantification methods. Here, we present DNA-origami-based fluorescence brightness standards for counting 5–300 copies of proteins in mammalian and bacterial cells, tagged with fluorescent proteins or organic dyes. Compared to conventional quantification techniques, our brightness standards are robust, straightforward to use, and compatible with nearly all fluorescence imaging applications, thereby providing a practical and versatile tool to quantify biomolecules via fluorescence microscopy. |
| Databáze: | OpenAIRE |
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