Troponin-I interacts with the met47 region of skeletal muscle actin. implications for the mechanism of thin filament regulation by calcium
Autor: | Terence Tao, Yin Luo, John Gergely, Bing Li, John D. Leszyk, Zhixing Li |
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Rok vydání: | 2002 |
Předmět: |
Arp2/3 complex
Hydroxylamine macromolecular substances Protein Engineering Peptide Mapping Methionine Structural Biology Troponin I medicine Animals Trypsin Amino Acid Sequence Actin-binding protein Muscle Skeletal Molecular Biology Chromatography High Pressure Liquid Actin Binding Sites biology Chemistry Actin remodeling Skeletal muscle musculoskeletal system Tropomyosin Molecular biology Actins Protein Structure Tertiary medicine.anatomical_structure Mutation biology.protein Biophysics Calcium Rabbits MDia1 Muscle Contraction Protein Binding |
Zdroj: | Journal of Molecular Biology. 316:429-434 |
ISSN: | 0022-2836 |
Popis: | Striated muscles are regulated by Ca 2+ via the thin filament proteins troponin (Tn) and tropomyosin (Tm). In the absence of Ca 2+ , contraction is inhibited, whereas myosin-actin interaction and contraction can take place in its presence. Although it is well established that the interaction of troponin-I (TnI), the inhibitory subunit of Tn, with actin is required for the inhibition process and that there are two separate actin-binding regions in TnI that interact with actin, the molecular mechanism of this inhibition process is still not clear. Using TnI mutants with photocrosslinking probes attached to genetically engineered cysteine residues in each of the two actin-binding regions, we show that both regions are close to Met47 of actin in its outer domain. It has been proposed that the Ca 2+ -induced activation of contraction involves the movement of Tm from the outer to the inner domain of the actin filament. On the basis of our results presented here, we propose that the position of Tm at the outer domain of actin in the Ca 2+ -free state is stabilized by the presence of TnI over actin’s outer domain via mutual interactions of all three components. In the presence of Ca 2+ , TnI’s actin-binding regions dissociate from actin allowing Tm to move toward actin’s inner domain. |
Databáze: | OpenAIRE |
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