Site of unequal sister chromatid exchange contains a potential Z-DNA-forming tract
| Autor: | Barbara K. Birshtein, Daniel R. Katzenberg, Ari Weinreb, Gary L. Gilmore |
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| Jazyk: | angličtina |
| Rok vydání: | 1988 |
| Předmět: |
Molecular Sequence Data
Sister chromatid exchange Biology DNA sequencing Cell Line chemistry.chemical_compound Mice Sequence Homology Nucleic Acid Gene duplication Animals Cloning Molecular Gene Southern blot Genetics Multidisciplinary Base Sequence Genes Immunoglobulin DNA Molecular biology chemistry Genes Immunoglobulin G Nucleic Acid Conformation Chromatid Immunoglobulin Constant Regions Immunoglobulin Heavy Chains Sister Chromatid Exchange Recombination Research Article Plasmacytoma |
| Popis: | Previous studies from this laboratory have provided evidence that the duplication of the IgG2a heavy chain constant region gene (C gamma 2a) in the murine myeloma cell line MPC-11 occurred via unequal sister chromatid exchange. We now report the determination of the DNA sequences of the germ-line regions in which this exchange has occurred. The two donor sequences have long regions of virtual identity. Furthermore, both chromatids contain stretches of T-C and T-G dinucleotides; the latter has the potential to form Z-DNA. Localization of the actual breakpoints via genomic Southern blot analysis suggests a novel mechanism for the recombination and strongly implicates the simple sequence in the process. |
| Databáze: | OpenAIRE |
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