Subacute ghrelin administration inhibits apoptosis and improves ultrastructural abnormalities in remote myocardium post-myocardial infarction
| Autor: | Fahaid Al-Hashem, Mahmoud A. Alkhateeb, Attalla F. El-kott, Refaat A. Eid, Mubarak Al-Shraim, Mohamed Samir Ahmed Zaki, Hoja Ibrahim, Hussain Aldera, Muhammad Alaa Eldeen, Samy M Eleawa |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Heart Ventricles Myocardial Infarction Apoptosis Caspase 3 030204 cardiovascular system & hematology Rats Sprague-Dawley Contractility 03 medical and health sciences 0302 clinical medicine Bcl-2-associated X protein Internal medicine medicine Animals Myocardial infarction bcl-2-Associated X Protein Pharmacology biology Chemistry Myocardium Hemodynamics General Medicine medicine.disease Ghrelin 030104 developmental biology Endocrinology medicine.anatomical_structure Ventricle Heart Function Tests biology.protein Ligation |
| Zdroj: | Biomedicine & Pharmacotherapy. 101:920-928 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2018.03.010 |
| Popis: | This study investigated the effect of ghrelin on cardiomyocytes function, apoptosis and ultra-structural alterations of remote myocardium of the left ventricle (LV) of rats, 21 days post myocardial infarction (MI). Rats were divided into 4 groups as a control, a sham-operated rats, a sham-operated+ghrelin, an MI + vehicle and an MI + ghrelin-treated rats. MI was induced by LAD ligation and then rats were recievd a concomitant doe of either normal saline as a vehicle or treated with ghrelin (100 μg/kg S.C., 2x/day) for 21 consecutive days. Ghrelin enhanced myocardial contractility in control rats and reversed the decreases in myocardial contractility and the increases in the serum levels of CK-MB and LDH in MI-induced rats. Additionally, it inhibited the increases in levels of Bax and cleaved caspase 3 and increased those for Bcl-2 in the remote myocardium of rat's LV, post-MI. At ultra-structural level, while ghrelin has no adverse effects on LV myocardium obtained from control or sham-treated rats, ghrelin post-administration to MI-induced rats reduced vascular formation, restored normal microfilaments appearance and organization, preserved mitochondria structure, and prevented mitochondrial swelling, collagen deposition and number of ghost bodies in the remote areas of their LV. Concomitantly, in remote myocardium of MI-induced rats, ghrelin enhanced endoplasmic reticulum intracellular organelles count, decreased number of atrophied nuclei and phagocytes, diminished the irregularity in the nuclear membranes and inhibited chromatin condensation. In conclusion, in addition to the physiological, biochemical and molecular evidence provided, this is the first study that confirms the anti-apoptotic effect of ghrelin in the remote myocardium of the LV during late MI at the level of ultra-structural changes. |
| Databáze: | OpenAIRE |
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