Simple and visible detection of duck hepatitis B virus in ducks and geese using loop-mediated isothermal amplification
| Autor: | Wu Qianqian, Xin Xu, Yingzuo Bi, Li Wanyu, Qingmei Xie, Wang Xueyu, Lu Yuan, Lunguang Yao, Yunchao Kan, Jun Ji |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
genetic structures
viruses Positive reaction Duck hepatitis B virus Loop-mediated isothermal amplification Cresol Red simplicity Sensitivity and Specificity Hepatitis B Virus Duck 03 medical and health sciences chemistry.chemical_compound DHBV Geese Animals Poultry Diseases 030304 developmental biology lcsh:SF1-1100 Phenol red 0303 health sciences biology Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Immunology Health and Disease Hepadnaviridae Infections biology.organism_classification visible detection 040201 dairy & animal science Molecular biology eye diseases Visual detection Real-time polymerase chain reaction Ducks Hepatitis Viral Animal Animal Science and Zoology Reaction system sense organs lcsh:Animal culture Nucleic Acid Amplification Techniques loop-mediated isothermal amplification |
| Zdroj: | Poultry Science, Vol 99, Iss 2, Pp 791-796 (2020) Poultry Science |
| ISSN: | 0032-5791 |
| Popis: | In this study, loop-mediated isothermal amplification (LAMP) was used to establish a rapid, specific, and visual detection method for duck hepatitis B virus (DHBV). The design and synthesis of 4 specific LAMP primers were based on the conserved gene region of the DHBV genome, and the optimum temperature and time of the LAMP reaction were 63°C and 50 min, respectively. The LAMP assay was confirmed to be specific for DHBV detection and had the same sensitivity as the quantitative PCR assay. A visual detection method for rapid determination of results was developed using a color indicator containing phenol red and cresol red. A color change was produced based on a pH change in the reaction system, indicating a positive reaction. For the detection of samples from ducks and geese, the LAMP method has the advantages of simplicity, high sensitivity and specificity, good visibility, and low cost. Moreover, it is more practical and convenient than PCR-related assays for the clinical detection of DHBV. |
| Databáze: | OpenAIRE |
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