High sperm aneuploidy rate in unselected infertile patients and its relationship with intracytoplasmic sperm injection outcome
| Autor: | Aldo E. Calogero, Adele De Palma, Nunziatina Burrello, Antonio Gulisano, I. Palermo, Nunziata Barone, Caterina Grazioso, Rosario D'Agata, Carlo Pafumi |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Infertility medicine.medical_specialty X Chromosome Sperm Injections medicine.medical_treatment Aneuploidy Biology Intracytoplasmic sperm injection Andrology Pregnancy Sperm Injections Intracytoplasmic Spermatozoa/ultrastructure Infertility Male/genetics Y Chromosome Male/genetics medicine Humans In Situ Hybridization Fluorescence Infertility Male reproductive and urinary physiology Gynecology Chromosomes Human Pair 12 urogenital system Rehabilitation Obstetrics and Gynecology medicine.disease Diploidy Spermatozoa Sperm Intracytoplasmic Abortion Spontaneous Pregnancy rate Treatment Outcome Reproductive Medicine Oligospermia Karyotyping Chromosome abnormality Female Chromosomes Human Pair 18 Chromosomes Human Pair 8 |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1460-2350 |
| Popis: | BACKGROUND: Men with oligoasthenoteratozoospermia (OAT) frequently undergo intracytoplasmic sperm injection (ICSI) as a treatment for their infertility. However, there is an increased risk of transmitting chromosomal abnormalities to the offspring given that natural selection is bypassed by the use of this technique and patients have an increased rate of sperm aneuploidy which, in addition, may negatively affect ICSI outcome. For this reason, the rate of sperm aneuploidy in unselected patients undergoing ICSI and its impact on ICSI performance have been evaluated. METHODS: Aneuploidy and diploidy were evaluated in spermatozoa separated by swim-up for oocyte injection, using DNA probes for chromosomes 8, 12, 18, X and Y. RESULTS: ICSI patients had sperm aneuploidy and diploidy rates significantly higher than those of 13 normozoospermic men who served as controls. Although the total aneuploidy rate varied considerably between the 18 patients, 15 of them (83%) had values above the upper range of the control group. Eighteen ICSI cycles were performed with an overall fertilization rate of 95% and a pregnancy rate of 39%. The aneuploidy rate of the 11 patients whose wives did not achieve pregnancy was slightly higher than that of pregnant couples, but the difference did not reach statistical significance. However, 10 patients in this group (91%) had a sperm aneuploidy rate well above the upper limit of the controls as compared with two patients in the 'pregnant' group (29%). CONCLUSIONS: This study has shown that unselected patients undergoing ICSI had an elevated sperm aneuploidy rate. Lack of pregnancy was associated with a tendency towards an increased aneuploidy rate; however, pregnancy occurred even in the presence of an elevated sperm aneuploidy rate. |
| Databáze: | OpenAIRE |
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