Cell type-oriented differential modulatory actions of saikosaponin-d on growth responses and DNA fragmentation of lymphocytes triggered by receptor-mediated and receptor-bypassed pathways
| Autor: | Masashi Kato, Ken-ichi Isobe, Mei-yi Pu, Izumi Nakashima, Taku Hattori, Noriyuki Yanagita |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Interleukin 2
Cellular immunity Sapogenins T-Lymphocytes Down-Regulation Thymus Gland Biology Lymphocyte Activation chemistry.chemical_compound Mice medicine Animals Oleanolic Acid Receptors Immunologic Receptor Interleukin 4 Calcimycin Cells Cultured Pharmacology DNA synthesis Antibodies Monoclonal Saponins Cell biology Up-Regulation Mice Inbred C57BL Thymocyte chemistry Immunology Phorbol DNA fragmentation Interleukin-2 Interleukin-4 Cell Division Spleen medicine.drug DNA Damage Signal Transduction |
| Zdroj: | Immunopharmacology. 29(3) |
| ISSN: | 0162-3109 |
| Popis: | We examined the immunoregulatory action of saikosaponin-d (SSd), which was isolated from the root of Bupleurum talcatum L. and had a steroid-like structure, on murine thymocytes, and compared the action with that on spleen cells. Constitutive DNA synthesis or the growth response stimulated with anti-CD3mAb of thymocytes were down-regulated by 3 micrograms/ml SSd, whereas with spleen cells these were up-regulated by the same concentration of SSd. On the other hand, 3 micrograms/ml of SSd greatly up-regulated the growth response and interleukin 2 (IL-2)/interleukin 4 (IL-4) production induced through a receptor-bypassed pathway by calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) in thymocytes, whereas it only slightly up-regulated them in spleen cells. Moreover, the same concentration of SSd inhibited DNA fragmentation in thymocytes induced by A23187 or PMA. These results suggest a unique cell type-dependent immuno-modulatory action of SSd. |
| Databáze: | OpenAIRE |
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