| Autor: |
Nadia Antonova, Khristo Khristov, Anika Alexandrova, Alexei Muravyov, Irena Velcheva |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Clinical hemorheology and microcirculation. |
| ISSN: |
1875-8622 |
| Popis: |
BACKGROUND AND OBJECTIVE: Microfluidics is a useful tool for investigating blood microrheology. The study aimed to present the development of a microfluidic device for assessing the microrheological properties of blood cells’ suspensions and its application in patients with diabetes mellitus type 2 (T2DM). METHODS: A new microfluidic device was elaborated, connected to a system, including a microscope with a digital camera, a pump with a manometer and a computer with specially developed software. Blood cells’ suspensions were investigated in a microchamber between two parallel optical slides within a 100μm distance. The motion of the blood cells in the microchamber was observed by the microscope and it was recorded and visualized by a digital camera. A method for evaluating the deformability of blood cells and a device for its implementation were used [1]. RESULTS: The pressure and flow rate ranges in the microfluidic device were specified by model suspensions of beta-ferroxy-hydroxide and red blood cells (RBC) suspensions. The pressure changes, realized by a pump (micropipette), connected to a manometer were established and the corresponding shear rates in the microfluidic device were determined. Data about the blood microrheological properties like RBC aggregation and deformability, leukocyte adhesion from a group of healthy volunteers and from patients with T2DM were obtained. CONCLUSIONS: The developed device and experimental system is a promising tool for the study of blood microrheology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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