Repeated Baclofen treatment ameliorates motor dysfunction, suppresses reflex activity and decreases the expression of signaling proteins in reticular nuclei and lumbar motoneurons after spinal trauma in rats
| Autor: | Jaroslav Pavel, Ján Gálik, Stefania Gedrova, Nadežda Lukáčová, Martin Marsala, Andrea Schreiberová, Ľudmila Hricová, Andrea Kucharíková, Monika Závodská |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Baclofen Histology Motor Activity chemistry.chemical_compound Internal medicine medicine Animals Spasticity Rats Wistar Spinal cord injury Spinal Cord Injuries Motor Neurons Neurons Glial fibrillary acidic protein biology business.industry musculoskeletal neural and ocular physiology Reticular Formation Lumbosacral Region Cell Biology General Medicine medicine.disease Spinal cord Immunohistochemistry Rats Reticulin Endocrinology medicine.anatomical_structure nervous system chemistry Gene Expression Regulation Anesthesia Reflex biology.protein Brainstem medicine.symptom Nitric Oxide Synthase business Parvalbumin Signal Transduction |
| Zdroj: | Acta histochemica. 116(2) |
| ISSN: | 1618-0372 |
| Popis: | The interruption of supraspinal input to the spinal cord leads to motor dysfunction and the development of spasticity. Clinical studies have shown that Baclofen (a GABA B agonist), while effective in modulating spasticity is associated with side-effects and the development of tolerance. The aim of the present study was to assess if discontinued Baclofen treatment and its repeated application leads antispasticity effects, and whether such changes affect neuronal nitric oxide synthase (nNOS) in the brainstem, nNOS and parvalbumin (PV) in lumbar α-motoneurons and glial fibrillary acidic protein in the ventral horn of the spinal cord. Adult male Wistar rats were exposed to Th9 spinal cord transection. Baclofen (30 mg/b.w.) diluted in drinking water, was administered for 6 days, starting at week 1 after injury and then repeated till week 4 after injury. The behavior of the animals was tested (tail-flick test, BBB locomotor score) from 1 to 8 weeks. Our results clearly indicate the role of nitric oxide, produced by nNOS in the initiation and the maintenance of spasticity states 1, 6 and 8 weeks after spinal trauma. A considerable decrease of nNOS staining after Baclofen treatment correlates with improvement of motor dysfunction. The findings also show that parvalbumin and astrocytes participate in the regulation of ion concentrations in the sub-acute phase after the injury. |
| Databáze: | OpenAIRE |
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