Proteomic Approach Identifies Alterations in Cytoskeletal Remodelling Proteins during Decidualization of Human Endometrial Stromal Cells
| Autor: | Andrew N. Stephens, Guiying Nie, Ying Li, Lynette M. Airey, Sarah Paule |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Proteomics
medicine.medical_specialty animal structures Stromal cell Biology Endometrium Biochemistry Mass Spectrometry SH3 domain Western blot Pregnancy Internal medicine Cyclic AMP medicine Humans Electrophoresis Gel Two-Dimensional Protein disulfide-isomerase Cells Cultured medicine.diagnostic_test Uterus Decidualization General Chemistry Tropomyosin Cell biology Cytoskeletal Proteins Endocrinology medicine.anatomical_structure Female Stromal Cells |
| Zdroj: | Journal of Proteome Research. 9:5739-5747 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr100525a |
| Popis: | Decidualization is a tissue remodelling process within the uterus in preparation for embryo implantation and pregnancy. In this study we isolated primary human endometrial stromal cells and stimulated decidualization with cAMP. We then used 2D- differential in-gel electrophoresis (DIGE) to identify proteins induced by decidualization. Eighty-eight out of 2714 spots were differentially regulated, 18 of which were assigned clear identities by mass spectrometry. Many of these are proteins known to be associated with cell structure and cytoskeletal remodelling. We validated five of these proteins by Western blot and immunohistochemistry on human endometrial tissue. The validated proteins are caldesmon 1, src substrate contactin 8, tropomyosin alpha-4 chain, protein disulfide isomerase 1A, and LIM and SH3 domain protein. With the exception of caldesmon 1, none of the identified proteins have previously been associated with decidualization. This study provides insight into our understanding of decidualization, which is important for successful embryo implantation and establishment of pregnancy. |
| Databáze: | OpenAIRE |
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