Conformational and Biological Properties of the Ala10 Analog of Human des-Trp1,Nle12-minigastrin
| Autor: | M Simonetti, Luis Moroder, Evaristo Peggion, Stefano Mammi, Erich Wünsch, Jean-Pierre Bali, Walter Göhring, M. T. Foffani, J C Galleyrand |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
Male
Conformational change Circular dichroism Magnetic Resonance Spectroscopy Chemical Phenomena Protein Conformation Stereochemistry Sequence (biology) Plasma protein binding Biochemistry Gastric Acid Protein structure Gastrins Animals Humans Alanine Tetrapeptide Chemistry Circular Dichroism Temperature Biological activity Nuclear magnetic resonance spectroscopy Rats Rabbits Protein Binding |
| Popis: | Synthesis, conformation, and biological properties of the Ala10 analogue of des-Trp1,Nle12-minigastrin are reported. Replacement of the Gly residue in the original sequence with Ala remarkably changes the conformational preference of the hormone in trifluoroethanol. CD and NMR results indicate that the conformational change is mainly located in the C-terminal portion of the molecule, with probable extension of the N-terminal alpha-helix throughout the entire sequence. The structural modification causes a 10-fold decrease in the biological potency of the hormone, which is about as active as the C-terminal tetrapeptide amide. These findings support our previous hypothesis that the optimal bioactive conformation of the native hormone is U-shaped, with mutual interactions among the two end segments. |
| Databáze: | OpenAIRE |
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