Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance

Autor: Francine Casse, Geneviève Conejero, Anne-Aliénor Véry, Shigetoshi Oiki, Hervé Sentenac, Pauline A. Essah, Nobuyuki Uozumi, Mark Tester, Cristina Savio, Thierry Simonneau, Aurélie Nublat, Pierre Berthomieu, William J. Brackenbury, Katsuyuki Yamada, Françoise Gosti, Cécile Lambert, Françoise Cellier
Přispěvatelé: Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Bioscience and Biotechnology Center, Nagoya University, Department of Physiology, Fukui Medical University, Fukui Medical University, Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Department of Plant Sciences (Cambridge, UK), University of Cambridge [UK] (CAM), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Unité mixte de recherche écophysiologie des plantes sous stress environnementaux, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Montpellier (ENSA M), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2003
Předmět:
0106 biological sciences
Xenopus
[SDV]Life Sciences [q-bio]
Arabidopsis
Beta-glucuronidase
Sodium Chloride
01 natural sciences
Cation Transport Proteins
ComputingMilieux_MISCELLANEOUS
Plant Proteins
0303 health sciences
Symporters
biology
General Neuroscience
food and beverages
BETA GLUCURONIDASE
Articles
Plants
Genetically Modified
Adaptation
Physiological
Cell biology
Biochemistry
Shoot
DNA
Complementary
Positional cloning
SALINITE DU SOL
Sodium
Molecular Sequence Data
chemistry.chemical_element
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
TOLERANCE AU SOL
Animals
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Amino Acid Sequence
Molecular Biology
DNA Primers
030304 developmental biology
Base Sequence
Sequence Homology
Amino Acid
General Immunology and Microbiology
Arabidopsis Proteins
fungi
biology.organism_classification
chemistry
Mutation
Symporter
Phloem
010606 plant biology & botany
Zdroj: EMBO Journal
EMBO Journal, EMBO Press, 2003, 22 (9), pp.2004-2014. ⟨10.1093/emboj/cdg207⟩
EMBO Journal, EMBO Press, 2003, 22 (9), pp.2004-2014
ISSN: 0261-4189
1460-2075
DOI: 10.1093/emboj/cdg207⟩
Popis: Two allelic recessive mutations of Arabidopsis, sas2-1 and sas2-2, were identified as inducing sodium overaccumulation in shoots. The sas2 locus was found (by positional cloning) to correspond to the AtHKT1 gene. Expression in Xenopus oocytes revealed that the sas2-1 mutation did not affect the ionic selectivity of the transporter but strongly reduced the macro scopic (whole oocyte current) transport activity. In Arabidopsis, expression of AtHKT1 was shown to be restricted to the phloem tissues in all organs. The sas2-1 mutation strongly decreased Na(+) concentration in the phloem sap. It led to Na(+) overaccumulation in every aerial organ (except the stem), but to Na(+) underaccumulation in roots. The sas2 plants displayed increased sensitivity to NaCl, with reduced growth and even death under moderate salinity. The whole set of data indicates that AtHKT1 is involved in Na(+) recirculation from shoots to roots, probably by mediating Na(+) loading into the phloem sap in shoots and unloading in roots, this recirculation removing large amounts of Na(+) from the shoot and playing a crucial role in plant tolerance to salt.
Databáze: OpenAIRE
Externí odkaz: