Murine Nr4a1 and Herpud1 are up-regulated by Wnt-1, but the homologous human genes are independent from β-catenin activation
| Autor: | Inko Nimmrich, Oliver Müller, Matthias Renner, Slava Chtarbova, Silke Erdmann, Peter Herter, Jan Kitajewski |
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| Rok vydání: | 2002 |
| Předmět: |
Beta-catenin
Transcription Genetic Nerve growth factor IB Morphogenesis Wnt1 Protein Biology Transfection Biochemistry Mice Transcription (biology) Proto-Oncogene Proteins Animals Humans Molecular Biology beta Catenin Wnt signaling pathway Cell Biology Zebrafish Proteins Blotting Northern Immunohistochemistry Molecular biology Up-Regulation Wnt Proteins Cytoskeletal Proteins Suppression subtractive hybridization Catenin Trans-Activators biology.protein Research Article Signal Transduction |
| Zdroj: | Biochemical Journal. 367:723-728 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | The Wnt signal transduction pathway regulates morphogenesis and mitogenesis of cells in multicellular organisms. A major downstream consequence of Wnt-1 signalling is the activation of β-catenin/T-cell factor (TCF)-mediated transcription. We compared Wnt-1-transformed murine mammary epithelial cells with control cells by subtractive hybridization. We found the two genes Nr4a1 and Herpud1 to be overexpressed in Wnt-1-transformed cells. Remarkably, the transcription levels of the two homologous human genes NR4A1 and HERPUD1 are neither activated in cells with activated β-catenin/TCF-mediated transcription nor can be induced by β-catenin transfection. These results indicate different regulation mechanisms of the two genes in murine and human cells. |
| Databáze: | OpenAIRE |
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