The novel high molecular weight Dermatophagoides farinae protein Zen-1 is a major allergen in North American and European mite allergic dogs with atopic dermatitis
| Autor: | Claude Favrot, Thierry Olivry, Stanley M. Dunston, Pascal Prélaud, Toshiroh Tsukui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Allergy 040301 veterinary sciences Enzyme-Linked Immunosorbent Assay Immunoglobulin E medicine.disease_cause Serology Dermatitis Atopic 0403 veterinary science Atopy 03 medical and health sciences Allergen Blood serum Dogs Antibody Specificity Mite Medicine Animals Antigens Dermatophagoides Dog Diseases General Veterinary biology business.industry fungi 04 agricultural and veterinary sciences Atopic dermatitis Intradermal Tests medicine.disease biology.organism_classification United States Europe 030104 developmental biology Immunology biology.protein Insect Proteins business |
| Zdroj: | Veterinary dermatology. 28(2) |
| ISSN: | 1365-3164 |
| Popis: | Background Atopic dogs with hypersensitivity to Dermatophagoides farinae (Df) have IgE recognizing high molecular weight (MW) allergens more often than the low MW Der f 1 and 2. A new high MW Df allergen, Zen-1, has been identified recently. Objectives To determine the IgE reactivity of American and European Df-hypersensitive dogs to Zen-1, Der f 1 and Der f 2. Methods We tested sera from 33 Df-reactive dogs from the USA, 29 from Europe and 15 experimentally sensitized to Df, by ELISA against crude Df, Der f 1, Der f 2 and Zen-1. ELISA inhibition was performed with sera reactive to Zen-1. Intradermal testing (IDT) was also done with the same allergens in 25 other American atopic dogs. Results Altogether, IgE seropositivity to Zen-1 was more prevalent (86%) than that to Der f 1 (17%) or Der f 2 (19%). The IgE reactivity to Zen-1 was correlated to that against crude Df; this allergen alone inhibited a high percentage (median: 50%; range: 22–84%) of the binding to the crude mite extract. The seropositivity to low MW allergens was highest in experimentally sensitized dogs. Serum IgE recognition of Der f 1 was low in dogs with AD; that to Der f 2 was significantly lower in American dogs (6%) than in European ones (28%). A high prevalence of positive immediate IDT reactions to Zen-1 confirmed the likely relevance of serological results. Conclusions and clinical importance This study establishes Zen-1 as a major allergen in atopic dogs sensitized to Df. |
| Databáze: | OpenAIRE |
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