Metabolic Activity and Monoclonal Antibody Production of Salmonella Enteritidis O and H Antigen Specific Hybridoma Cells in Static Culture
| Autor: | Kerem Bora, Ayse Nalbantsoy, I Deliloğlu-Gürhan |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Cell Survival
medicine.drug_class Glutamine Salmonella enteritidis Immunology Cell Culture Techniques chemical and pharmacologic phenomena Biology H antigen Monoclonal antibody Cell Line Antigen Ammonia medicine Immunology and Allergy Lactic Acid Monoclonal antibody production Antigens Bacterial Hybridomas Antibodies Monoclonal Molecular biology Culture Media Kinetics Hybridoma cell Glucose Salmonella Infections Metabolic activity |
| Zdroj: | Hybridoma. 30:189-193 |
| ISSN: | 1557-8348 1554-0014 |
| DOI: | 10.1089/hyb.2010.0085 |
| Popis: | The aim of the present work was to study the kinetics of two hybridomas that produce monoclonal antibody against Salmonella Enteritidis O (5A8) and H (D7) antigen. The hybridomas originated from the Ag8x653 (5A8) and Sp2/O (D7) myeloma cell lines. The relationship between the uptake of glucose and glutamine and the release of the lactate and ammonia and monoclonal antibody production into hybridoma growth were investigated in static culture with serum-containing DMEM/F:12 medium for the determination of pilot-production strategies of the hybridomas. Results showed that glucose and glutamine concentrations were reduced, with an increase in ammonia and lactate concentration in the culture medium. The hybridoma cell line 5A8 has shown lower metabolic activities compared with D7, whereas its monoclonal antibody productivity was found to be two-fold higher than the D7. MAb production by the hybridoma cell line 5A8 seems promising, considering the moderate level of productivity compared to that found in the literature. |
| Databáze: | OpenAIRE |
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