Humoral and cellular immune responses in sheep immunized with a 22 kilodalton exported protein of Mycobacterium avium subspecies paratuberculosis
| Autor: | Rachael C. Rigden, Brigitte Gicquel, Nicolas Lopez-Villalobos, Chris Dupont, Norihiro Maeda, Dianna Crosbie-Caird, Dakshina M. Jandhyala, Alan Murray |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Microbiology (medical) Cellular immunity Injections Subcutaneous Lipoproteins Blotting Western Microbiology law.invention Interferon-gamma Immune system Adjuvants Immunologic Bacterial Proteins Western blot Antibody Specificity law Escherichia coli medicine Animals Tuberculosis Vaccines Synthetic Sheep biology medicine.diagnostic_test Immunogenicity Water General Medicine biology.organism_classification Antibodies Bacterial Virology Mycobacterium avium subspecies paratuberculosis Recombinant Proteins Molecular Weight Bacterial Vaccines Humoral immunity biology.protein Recombinant DNA Immunization Antibody Oils Mycobacterium avium |
| Zdroj: | Journal of Medical Microbiology. 55:1735-1740 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.46785-0 |
| Popis: | An immunogenic 22 kilodalton exported Mycobacterium avium subspecies paratuberculosis (MAP) lipoprotein (P22) was previously identified, and found to belong to the LppX/LprAFG family of mycobacterial lipoproteins. N-terminal polyhistidine-tagged P22 was produced and purified from Escherichia coli. Antibody recognition of P22, and interferon-gamma (IFN-γ) responses in vitro using blood from a sheep vaccinated with Neoparasec, confirmed its immunogenicity. To evaluate the immunogenicity of P22 in vivo, five sheep were immunized with a single dose containing 0.8 mg recombinant P22 protein in adjuvant. Blood was collected at 4, 13 and 29 weeks post-immunization (p.i.) and tested for anti-P22 antibodies and P22-specific IFN-γ production. P22-specific antibodies were detected by Western blot analysis in all five Neoparasec-immunized sheep at the three time points. Three out of five P22-immunized sheep produced P22-specific antibodies for up to 13 weeks p.i., and two gave a response at 29 weeks p.i. Recombinant P22 was able to stimulate significant IFN-γ production in blood of P22-immunized sheep at 13 and 29 weeks p.i. Recombinant P22 also elicited an IFN-γ response in blood of sheep immunized with Neoparasec. |
| Databáze: | OpenAIRE |
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