Reference limits for GAD65 and IA-2 autoantibodies by chemiluminescence immunoassay in Northern European adults and children

Autor:	Cindy Soendersoe Knudsen, Agnes Ziobrowska-Bech, Britta Kremke, Anne Winther-Larsen, Tina Parkner
Rok vydání:	2019
Předmět:	Male Luminescence endocrine system diseases Chemiluminescence immunoassay Clinical Biochemistry Gene Expression 01 natural sciences 0302 clinical medicine immune system diseases Reference Values Healthy volunteers antibodies 030212 general & internal medicine Child Immunoassay biology Glutamate Decarboxylase General Medicine Middle Aged Healthy Volunteers Europe Child Preschool diabetes mellitus Female Antibody Adult Adolescent Immunoassay/standards European Continental Ancestry Group White People Glutamate Decarboxylase/antagonists & inhibitors paediatrics 03 medical and health sciences Diabetes mellitus medicine Humans Receptor-Like Protein Tyrosine Phosphatases Class 8 Aged Autoantibodies Type 1 diabetes Receptor-Like Protein Tyrosine Phosphatases Class 8/antagonists & inhibitors ICA512 autoantibody business.industry 010401 analytical chemistry Autoantibody Infant Newborn nutritional and metabolic diseases Infant Diabetes Mellitus Type 1/blood medicine.disease 0104 chemical sciences Diabetes Mellitus Type 1 Reference values GAD65 Immunology biology.protein business Autoantibodies/blood Biomarkers/blood Biomarkers
Zdroj:	Ziobrowska-Bech, A, Winther-Larsen, A, Kremke, B, Parkner, T & Soendersoe Knudsen, C 2019, ' Reference limits for GAD65 and IA-2 autoantibodies by chemiluminescence immunoassay in Northern European adults and children ', Scandinavian Journal of Clinical and Laboratory Investigation, vol. 79, no. 1-2, pp. 123-125 . https://doi.org/10.1080/00365513.2019.1566566
ISSN:	1502-7686
DOI:	10.1080/00365513.2019.1566566
Popis:	The GAD65 and IA-2 antibodies (Abs) are biomarkers of the development of type 1 diabetes mellitus (T1DM) in both children and adults. The upper reference limit for the autoantibodies made by the manufacture was established on an adult Chinese population. Here, we established upper reference limits for Northern European adults and children in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Serum samples from healthy Danish children (0–18 years) and adults (18–70 years) were analysed for GAD65Ab and IA-2Ab using MAGLUMI 800 Chemiluminescence Immunoassay (CLIA). The Kruskal–Wallis test was used for evaluating differences between gender and age groups. No gender or age differences were found for neither GAD65Ab nor IA-2Ab, and a combined upper reference limit for both children and adults could be established. An upper reference limit of 5.1 IU/mL was defined for GAD65Ab and 11.5 U/mL for IA-2Ab. Our results showed a substantial discrepancy with the reference limits established by the manufacturer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::616b46c7ded559cbb1d0df8f9643549e https://pubmed.ncbi.nlm.nih.gov/30727763 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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