Birth of cloned calves from vitrified–warmed zona-free buffalo (Bubalus bubalis) embryos produced by hand-made cloning
Autor: | Radhey S. Manik, Sudeepta Kumar Panda, Prabhat Palta, Manmohan Singh Chauhan, Suresh Kumar Singla, Ambikaprasanna Saha |
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Rok vydání: | 2013 |
Předmět: |
medicine.medical_specialty
Buffaloes Cloning Organism Theriogenology Embryonic Development India Reproductive technology Biology Cryopreservation Andrology Fetus Endocrinology Pregnancy Genetics medicine Animals Blastocyst Molecular Biology Cells Cultured reproductive and urinary physiology Gametogenesis Skin Ear Embryo culture Embryo Anatomy Fibroblasts Embryo Transfer Embryo Mammalian Vitrification In Vitro Oocyte Maturation Techniques Transgenesis medicine.anatomical_structure Animals Newborn Reproductive Medicine embryonic structures Female Animal Science and Zoology Live Birth Developmental Biology Biotechnology |
Zdroj: | Reproduction, Fertility and Development. 25:860 |
ISSN: | 1031-3613 |
DOI: | 10.1071/rd12061 |
Popis: | The availability of techniques for the vitrification of cloned blastocysts can improve their effective use. The present study compared the developmental competence of buffalo cloned embryos derived from adult (BAF), newborn (BNF) and fetal fibroblast (BFF) before and after vitrification. Despite similar cleavage rates among the three groups, the blastocyst rate was lower for BAF- than BNF- and BFF-derived embryos (30.2 ± 2.2% vs 41.7 ± 1.7% and 39.1 ± 2.1%, respectively; P |
Databáze: | OpenAIRE |
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