Replication of plasmid-derived human papillomavirus type 11 DNA in cultured keratinocytes
| Autor: | Bettie M. Steinberg, S Mungal, L B Taichman |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
DNA Replication
Keratinocytes viruses Immunology Biology Transfection Microbiology Virus chemistry.chemical_compound Foreskin Plasmid Virology medicine Humans Serial Passage Papillomaviridae Cells Cultured DNA replication biology.organism_classification Molecular biology Tumor Virus Infections medicine.anatomical_structure chemistry Insect Science DNA Viral Keratinocyte DNA Research Article Plasmids |
| Zdroj: | Journal of Virology. 66:3220-3224 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.66.5.3220-3224.1992 |
| Popis: | Plasmid-derived human papillomavirus type 11 (HPV11) DNA has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes. HPV11 DNA was excised from the bacterial plasmid, religated to form circular molecules, and cotransfected along with pSV2Neo. Transfectants were selected and shown to contain replicated episomal HPV DNA. Once selected, HPV11 DNA persists in cells through at least two additional passages. |
| Databáze: | OpenAIRE |
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