Physiochemical characterization and cytotoxicity evaluation of mercury-based formulation for the development of anticancer therapeuticals

Autor: Arul Amuthan, S. Shanmuga Sundar, N. Kannan, S. Balaji, Natesan Balasubramanian, N. V. Anil Kumar
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Embryology
Cytotoxicity
Developmental toxicity
Cancer Treatment
lcsh:Medicine
Pharmacology
Toxicology
Pathology and Laboratory Medicine
030226 pharmacology & pharmacy
0302 clinical medicine
Spectrum Analysis Techniques
Medicine and Health Sciences
Enzyme assays
Colorimetric assays
lcsh:Science
Bioassays and physiological analysis
Zebrafish
Multidisciplinary
MTT assay
Chemistry
Eukaryota
Animal Models
Experimental Organism Systems
Oncology
Osteichthyes
Toxicity
Vertebrates
Cell lines
Biological cultures
Research Article
Research and Analysis Methods
03 medical and health sciences
Model Organisms
In vivo
Animals
HEK 293 cells
lcsh:R
Embryos
Organisms
Correction
Biology and Life Sciences
X-Ray Photoelectron Spectroscopy
In vitro
030104 developmental biology
Fish
Cell culture
Biochemical analysis
lcsh:Q
Electron Beam Spectrum Analysis Techniques
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 4, p e0195800 (2018)
ISSN: 1932-6203
Popis: Background The present study is aimed to evaluate the physiochemical properties and cytotoxicity of mercury-based formulation for the development of anticancer therapeuticals. Methods The elemental and morphological features of the formulation were characterized by FE-SEM, XPS and EDS. The described formulation was evaluated for its cytotoxicity on Hek293 and MCF7 cell lines using MTT assay to study the in vitro effects. The in vivo developmental toxicity was also studied on zebrafish embryos and the lethal concentration (LC50) values were calculated as per the OECD regulations. Results The elemental and morphological characterizations confirmed the presence of mercuric compounds. The particles were spherical and stable with the size ranges between 20 and 80nm. Although the PK formulation contains mercurials it was very effective only to cancerous cells (MCF-7) and it is less toxic to normal cells (HEK 293). The in vivo assessment of developmental toxicity on zebrafish embryo confirmed the safer dosage of 100μg/ml. However, a higher dosage of 1mg/ml led to the malformation of embryos such as pericardial, tail and yolk sac edema. Conclusion The physiochemical characterization of PK formulation confirmed the presence of HgS. The results of both in vitro and in vivo studies showed that the formulation is less toxic. Although the test sample contains mercurials it was very effective against cancerous cells (MCF-7) and it is less toxic to normal cells (HEK 293). Future studies Further studies on effectiveness of the formulation along with inflammatory response in mice models are to be conducted.
Databáze: OpenAIRE
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