Analysis of the Fam181 gene family during mouse development reveals distinct strain-specific expression patterns, suggesting a role in nervous system development and function
| Autor: | Bernhard G. Herrmann, Lisette Lange, Lars Wittler, Tracie Pennimpede, Phillip Grote, Matthias Marks |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cellular differentiation Embryonic Development Nerve Tissue Proteins Biology Cell Line 03 medical and health sciences Mice Biological Clocks Somitogenesis Gene expression Genetics Paraxial mesoderm Gene family Animals Humans TEAD4 Gene Embryonic Stem Cells Phylogeny Receptors Notch Lateral plate mesoderm fungi Gene Expression Regulation Developmental Cell Differentiation General Medicine 030104 developmental biology HEK293 Cells Somites Multigene Family Signal Transduction |
| Zdroj: | Gene |
| ISSN: | 1879-0038 |
| Popis: | During somitogenesis differential gene expression can be observed for so-called cyclic genes, which display expression changes with a periodicity of 120 min in the mouse. In screens to identify novel cyclic genes in murine embryos, Fam181b was predicted to be an oscillating gene in the presomitic mesoderm (psm). This gene, and its closely related paralog Fam181a, belong to the thus far uncharacterized Fam181 gene family. Here we describe the expression of Fam181b and Fam181a during murine embryonic development. In addition, we confirm oscillation of Fam181b in the psm in-phase with targets of, and regulated by, Notch signaling. Fam181b expression in the psm, as well as in the lateral plate mesoderm, was found to be affected by genetic background. We show that Fam181a and b exhibit partially overlapping mRNA expression patterns, and encode for proteins containing highly-conserved motifs, which predominantly localize to the nucleus. A Fam181b loss-of-function model was generated and found to result in no obvious phenotype. |
| Databáze: | OpenAIRE |
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