Pulsed-field gel electrophoresis as an epidemiological tool for clonal identification ofAeromonas hydrophila
| Autor: | Y. Michel-Briand, Daniel Talon, J. Lesne, M J Dupont, Michelle Thouverez |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Gel electrophoresis Genotype medicine.drug_class Antibiotics DNA Restriction Enzymes biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses biology.organism_classification Applied Microbiology and Biotechnology Microbiology Aeromonas hydrophila Anti-Bacterial Agents Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Vibrionaceae Pulsed-field gel electrophoresis medicine Typing Restriction fragment length polymorphism |
| Zdroj: | Journal of Applied Bacteriology. 80:277-282 |
| ISSN: | 0021-8847 |
| Popis: | Pulsed-field gel electrophoresis (PFGE) was used to characterize Aeromonas hydrophila strains isolated from a cluster of hospital-acquired infections that occurred over approximately 1 month in a French hospital. Five isolates from patients and 10 isolates from the water supply were characterized by biotyping and antibiotic susceptibility patterns and compared with 10 epidemiologically unrelated strains isolated from patients and rivers, by PFGE of digests of chromosomal DNA. Five environmental and four clinical isolates belonged to the same biotype and antibiotic susceptibility pattern type. The endonucleases XbaI, SpeI and SwaI gave satisfactory profiles whereas DraI did not. The profiles were stable, reproducible and discriminatory. The 10 epidemiologically unrelated strains exhibited 10 different patterns after digestion with XbaI, the least expensive, suitable endonuclease. PFGE is a rapid and discriminatory technique for the typing of Aeromonas hydrophila where a common origin of infection is suspected. |
| Databáze: | OpenAIRE |
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