Impact of sample storage on detection of periodontal bacteria
| Autor: | Marianne Weibel, Joannis Katsoulis, L. J. R. Heitz-Mayfield, Niklaus P. Lang, Regula Hirschi, G. R. Persson |
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| Rok vydání: | 2005 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Preservation Biological Immunology Dental Plaque Pilot Projects Microbiology Statistics Nonparametric Humans Tannerella forsythia Food science Periodontitis General Dentistry Analysis of Variance biology Nucleic Acid Hybridization Campylobacter rectus Treponema denticola biology.organism_classification stomatognathic diseases Checkerboard Actinobacillus Linear Models Fusobacterium nucleatum Bacteria Campylobacter gracilis |
| Zdroj: | Oral Microbiology and Immunology. 20:128-130 |
| ISSN: | 1399-302X 0902-0055 |
| Popis: | Background/aims: Information on the impact of sample storage prior to analysis by DNA methods is limited. The aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration. Material and methods: Subgingival plaque samples were studied by a) checkerboard DNA–DNA hybridization by immediate processing, b) storage at + 4°C for 6 weeks, c) storage at − 20°C for 6 months or d) storage at − 20°C for 12 months. Results: No differences in total DNA were found between protocol 1 and 2, or between protocol 3 and 4. Protocol 1 yielded 2.4 times more total bacterial DNA than did protocol 3 (P |
| Databáze: | OpenAIRE |
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